Difference between revisions of "Part:BBa K779500:Design"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K779500 short</partinfo>
 
<partinfo>BBa_K779500 short</partinfo>
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===Design Notes===
 
===Design Notes===
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Sequence designed to be orthogonal to the HEK293 transcriptome, and to have a high melting temperature.
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4-nucleotide bulge added to BBa_K779501. Bulge placed 10-nt from the 3' end as a potential protection mechanism from cleavage by RISC as suggested by Iba et al. (2011).
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4-nucleotide bulge added 10-nt from the 3' end as a potential protection mechanism from cleavage by RISC as suggested by Iba et al. (2011).
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See more details at the MIT iGEM wiki: http://2012.igem.org/Team:MIT
 
See more details at the MIT iGEM wiki: http://2012.igem.org/Team:MIT
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===Source===
 
===Source===

Revision as of 20:18, 29 September 2012

Long reporter top strand with bulge MammoBlock


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

4-nucleotide bulge added to BBa_K779501. Bulge placed 10-nt from the 3' end as a potential protection mechanism from cleavage by RISC as suggested by Iba et al. (2011).

See more details at the MIT iGEM wiki: http://2012.igem.org/Team:MIT

Source

De novo design.

References