Difference between revisions of "Part:BBa K776020:Experience"

 
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
 
This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
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===Applications of BBa_K776020===
 
===Applications of BBa_K776020===
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== '''iGEM CINVESTAV_IPN-UNAM''' ==
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The genetic regulation system AppA/PpsR light dependent was used in purple non-sulfur photosynthetic bacteria (PNSP) ''R. sphaeroides'' and  ''R. palustris'', using different conditions. For test the  system we used as a reporter GFP.
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[[Image:system1.jpg]]
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''Fig 1. Construction of the genetic regulation system AppA/PpsR light dependent.''
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We employed flow cytometery for mesuring the expression of GFP. The conditions we used for test genetic regulation system AppA/PpsR light dependent were: aerobic/darness, anaerobic/light, anaerobic/darknness. The results were as follows.
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'''''R. sphaeroides'''''
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[[Image:a1.jpg]]
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''Graphic 1.: Quantitative analysis of bacterial population with GFP expression (GFP+), under above condition, using flow cytometry (Attune cytometer Applied Biosystems), background signals were eliminated using a negative control.''
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[[Image:22.jpg]]
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''Graphic 2. Median fluorescence Intensity of the conjugated R. sphaeroides strain. Analysis of fluorescence in bacterial populations (1000 bacteria), under above conditions, using flow cytometry (Attune cytometer Applied Biosystems), background signals were eliminated using a negative control.''
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'''''R. palustris'''''
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[[Image:23.jpg]]
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''Graphic 3.: Quantitative analysis of bacterial population with GFP expression (GFP+), under above condition, using flow cytometry (Attune cytometer Applied Biosystems), background signals were eliminated using a negative control.''
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[[Image:24.jpg]]
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''Graphic 4. Median fluorescence Intensity of the conjugated R. palustris strain. Analysis of fluorescence in bacterial populations (1000 bacteria), under above conditions, using flow cytometry (Attune cytometer Applied Biosystems), background signals were eliminated using a negative control.''
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Results above shows that our system is functional in our two PNSP bacteria.
  
 
===User Reviews===
 
===User Reviews===

Latest revision as of 20:16, 29 September 2012

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K776020

iGEM CINVESTAV_IPN-UNAM

The genetic regulation system AppA/PpsR light dependent was used in purple non-sulfur photosynthetic bacteria (PNSP) R. sphaeroides and R. palustris, using different conditions. For test the system we used as a reporter GFP.

System1.jpg

Fig 1. Construction of the genetic regulation system AppA/PpsR light dependent.


We employed flow cytometery for mesuring the expression of GFP. The conditions we used for test genetic regulation system AppA/PpsR light dependent were: aerobic/darness, anaerobic/light, anaerobic/darknness. The results were as follows.



R. sphaeroides

A1.jpg

Graphic 1.: Quantitative analysis of bacterial population with GFP expression (GFP+), under above condition, using flow cytometry (Attune cytometer Applied Biosystems), background signals were eliminated using a negative control.


22.jpg

Graphic 2. Median fluorescence Intensity of the conjugated R. sphaeroides strain. Analysis of fluorescence in bacterial populations (1000 bacteria), under above conditions, using flow cytometry (Attune cytometer Applied Biosystems), background signals were eliminated using a negative control.



R. palustris

23.jpg

Graphic 3.: Quantitative analysis of bacterial population with GFP expression (GFP+), under above condition, using flow cytometry (Attune cytometer Applied Biosystems), background signals were eliminated using a negative control.


24.jpg

Graphic 4. Median fluorescence Intensity of the conjugated R. palustris strain. Analysis of fluorescence in bacterial populations (1000 bacteria), under above conditions, using flow cytometry (Attune cytometer Applied Biosystems), background signals were eliminated using a negative control.


Results above shows that our system is functional in our two PNSP bacteria.

User Reviews

UNIQ3ace8c7a23f549c3-partinfo-00000000-QINU UNIQ3ace8c7a23f549c3-partinfo-00000001-QINU