Difference between revisions of "Part:BBa K864100:Experience"

Revision as of 18:30, 29 September 2012

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Applications of BBa_K864100

User Reviews

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iGEM Team Uppsala University 2012

This part was used as an fluorescent reporter in two ways by the Uppsala iGEM team 2012. It could easily be observed both in a Fluorescence activated cell sorter (FACS), on a UV table and even better on a Visi-Blue blue-light table. We recommend it whenever a strong fluorescent reporter is needed.

1. As a part of the sRNA screening system target construct (BBa_K864444), where it was a reporter of translational downregulation. Thanks to the very strong fluorescence, cells with very low levels of expression could easily be distinguished from non-fluorescent cells, using a FACS. In this experiment it was fused to a part of another protein, with the BBa_J18922 gly-ser linker in between. Fluorescence was not affected by this fusion.

2. In our promoter test, where it was coupled to different promoter as promoter-B0032-SYFP2 (using BBa_K864101).

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 This part was used as an fluorescent reporter in two ways by the Uppsala iGEM team 2012. It could easily be observed both in a Fluorescence activated cell sorter (FACS), on a UV table and even better on a Visi-Blue blue-light table. We recommend it whenever a strong fluorescent reporter is needed.
-. As a part of the sRNA screening system target construct (<partinfo>K864444</partinfo>), where it was a reporter of translational downregulation. Thanks to the very strong fluorescence, cells with very low levels of expression could easily be distinguished from non-fluorescent cells, using a FACS. In this experiment, it was fused to a part of another protein, with the <partinfo>J18922</partinfo> gly-ser linker in between. Fluorescence was not affected by this fusion.
+. As a part of the sRNA screening system target construct (<partinfo>K864444</partinfo>), where it was a reporter of translational downregulation. Thanks to the very strong fluorescence, cells with very low levels of expression could easily be distinguished from non-fluorescent cells, using a FACS. In this experiment it was fused to a part of another protein, with the <partinfo>J18922</partinfo> gly-ser linker in between. Fluorescence was not affected by this fusion.
 . In our promoter test, where it was coupled to different promoter as promoter-B0032-SYFP2 (using <partinfo>K864101</partinfo>).