Difference between revisions of "Part:BBa K845000"
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===Functional Parameters=== | ===Functional Parameters=== | ||
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Revision as of 16:40, 29 September 2012
iGEM 2012 Grenoble Team proposes a new design and application to the pBAD promoter(paraBAD).
In order to create a biological AND gate the 2012 Grenoble team worked on the cAMP-CRP complex and arabinose inductible promoter.
The activity of the promoter was tested under several conditions ( http://2012.igem.org/Team:Grenoble/Biology/Protocols/AND_test ) using a GFP as a reporter (on a pUA66). We tested this activity in BW25113 ΔcyaA in different culture media (Glucose + Acetate; Glycerol; Acetate).
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 500
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 344
Illegal XhoI site found at 494 - 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 500
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 500
Illegal AgeI site found at 179
Illegal AgeI site found at 484 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1169
Illegal SapI site found at 161
Functional Parameters
| emission | 504 nm |
| excitation | 395 and 475 nm |
| negative_regulators | AraC protein |
| positive_regulators | cAMP-CRP complex and Arabinose |