Difference between revisions of "Part:BBa K786010:Experience"
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | ||
how you used this part and how it worked out. | how you used this part and how it worked out. | ||
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| + | === Characterization of BBa_K786010=== | ||
| + | <p><strong>I. biobrick DNA length verification</strong></p> | ||
| + | <p>In order to check the inserted biobrick DNA length, restriction digestion was performed by EcoRI and PstI, targeting on biobrick standard RFC10 site.</p> | ||
| + | https://static.igem.org/mediawiki/2012/a/a7/Chara4 | ||
===Applications of BBa_K786010=== | ===Applications of BBa_K786010=== | ||
| + | Together with, Negative Phototactic Construct for Blue Light Detection (BBa_K786001) Positive Phototactic Construct for Blue Light Detection (BBa_K786002), Phototactic Construct for Orange Light Detection (BBa_K786003) and the genome targeting CRISPR/Cas systems BBa_K786031. This part will have the following application. | ||
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| + | Incorporation with BBa_K786031 as a new biosafety approach | ||
| + | https://static.igem.org/mediawiki/2012/a/a5/Appp5.png | ||
| + | <p>Genetically-modified organisms have been put in use extensively throughout the last decade. Yet, concerns about such organisms causing undesirable effect to the environment once released have been raised. Our biobricks might be one of the answers to tackle such concerns. When the gene expression system for sensory rhodopsin is incorporated with the CRISPR/Cas system, bio-engineered cells would cleave their own DNA into nucleotides once exposed to natural light. <br /> | ||
| + | This system would be useful for bio-engineered cells that could be used to work in the dark. (e.g.: Biofuel synthesizing cells, human hormone-producing cells)</p> | ||
===User Reviews=== | ===User Reviews=== | ||
Revision as of 16:06, 29 September 2012
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Characterization of BBa_K786010
I. biobrick DNA length verification
In order to check the inserted biobrick DNA length, restriction digestion was performed by EcoRI and PstI, targeting on biobrick standard RFC10 site.
https://static.igem.org/mediawiki/2012/a/a7/Chara4
Applications of BBa_K786010
Together with, Negative Phototactic Construct for Blue Light Detection (BBa_K786001) Positive Phototactic Construct for Blue Light Detection (BBa_K786002), Phototactic Construct for Orange Light Detection (BBa_K786003) and the genome targeting CRISPR/Cas systems BBa_K786031. This part will have the following application.
Incorporation with BBa_K786031 as a new biosafety approach
Genetically-modified organisms have been put in use extensively throughout the last decade. Yet, concerns about such organisms causing undesirable effect to the environment once released have been raised. Our biobricks might be one of the answers to tackle such concerns. When the gene expression system for sensory rhodopsin is incorporated with the CRISPR/Cas system, bio-engineered cells would cleave their own DNA into nucleotides once exposed to natural light.
This system would be useful for bio-engineered cells that could be used to work in the dark. (e.g.: Biofuel synthesizing cells, human hormone-producing cells)
User Reviews
UNIQ0293ca6bda28217c-partinfo-00000000-QINU UNIQ0293ca6bda28217c-partinfo-00000001-QINU