Difference between revisions of "Part:BBa K845000:Experience"

Revision as of 15:23, 29 September 2012

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Applications of BBa_K845000

In order to characterize the paraBAD promoter we used a transcriptional fusion of the promoter and gfpmut2 from Alon on pUA66 in BW25113 ΔcyaA. We followed the fluorescent expression of the GFP versus different concentrations of arabinose AND cAMP over time. We made this experiment in different growth media (the different media did not affect bacterial growth):

M9 complement with 0.03% of glucose and 0.03% of acetate (1)

M9 complement with 0.1% of glycerol (2)

M9 complement with 0.1% of acetate (3)

See the protocol: http://2012.igem.org/Team:Grenoble/Biology/Protocols/AND_test

Robustness test of the AND gate:

User Reviews

UNIQ970991d5e4085419-partinfo-00000000-QINU UNIQ970991d5e4085419-partinfo-00000001-QINU

@@ Line 6: / Line 6: @@
 In order to characterize the paraBAD promoter we used a transcriptional fusion of the promoter and gfpmut2 from Alon on pUA66 in BW25113 ΔcyaA.
-We followed the fluorescent expression of the GFP versus different concentrations of arabinose AND cAMP over time. We made this experiment in different growth media:
+We followed the fluorescent expression of the GFP versus different concentrations of arabinose AND cAMP over time. We made this experiment in different growth media (the different media did not affect bacterial growth):
 M9 complement with 0.03% of glucose and 0.03% of acetate (1)
@@ Line 17: / Line 17: @@
 See the protocol: http://2012.igem.org/Team:Grenoble/Biology/Protocols/AND_test
-Those different media did not affect bacterial growth.
+Robustness test of the AND gate:
 [[Image:Characterization_glucose.png]]