Difference between revisions of "Part:BBa K772009:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | Note that there is an RBS sequence just before the protein sequence in order to eliminate the need of addition of it. | + | Note that there is an RBS sequence just before the protein sequence in order to eliminate the need of addition of it. More on that, before the GFP section, there is a flag tag in order to isolate this protein component and use it in the Co-IP assay. |
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===Source=== | ===Source=== | ||
Latest revision as of 13:27, 29 September 2012
GFP - LasR protein complex with flag tags
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1180
Illegal AgeI site found at 1377 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 693
Design Notes
Note that there is an RBS sequence just before the protein sequence in order to eliminate the need of addition of it. More on that, before the GFP section, there is a flag tag in order to isolate this protein component and use it in the Co-IP assay.
Source
Cleavage site, LasR and GFP sequences are gathered from Registry.