Difference between revisions of "Part:BBa K772008:Design"

 
 
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<partinfo>BBa_K772008 short</partinfo>
 
<partinfo>BBa_K772008 short</partinfo>
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===Design Notes===
 
===Design Notes===
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Cleavage site, LacZ and GFP sequences are gathered from Registry.
 
  
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Note that there is an RBS sequence just before the protein sequence in order to eliminate the need of addition of it. More on that, before the GFP section, there is a flag tag in order to isolate this protein component and use it in the Co-IP assay.
  
  
 
===Source===
 
===Source===
  
−
Note that there is an RBS sequence just before the protein sequence in order to eliminate the need of addition of it.  
+
Cleavage site, LacZ and GFP sequences are gathered from Registry.
  
 
===References===
 
===References===

Latest revision as of 13:21, 29 September 2012

GFP - LacZ protein complex with flag tags


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 693


Design Notes

Note that there is an RBS sequence just before the protein sequence in order to eliminate the need of addition of it. More on that, before the GFP section, there is a flag tag in order to isolate this protein component and use it in the Co-IP assay.


Source

Cleavage site, LacZ and GFP sequences are gathered from Registry.

References