Difference between revisions of "Part:BBa K772005:Design"
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===Design Notes=== | ===Design Notes=== | ||
Note that there is an RBS sequence just before LasI; in order to be easily used with quorum sensing receiver devices such as BBa_F2620 or BBa_K772004. | Note that there is an RBS sequence just before LasI; in order to be easily used with quorum sensing receiver devices such as BBa_F2620 or BBa_K772004. | ||
| − | + | Moreover, LacI sequence is added in order to regulate the whole part in the assays by using IPTG tp stop the inhibition of LacI. To do this, pLac promoter with RBS (e.g. J04500) must be assembled. | |
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===Source=== | ===Source=== | ||
Latest revision as of 11:12, 29 September 2012
AHL generator: LasI
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1581
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Note that there is an RBS sequence just before LasI; in order to be easily used with quorum sensing receiver devices such as BBa_F2620 or BBa_K772004. Moreover, LacI sequence is added in order to regulate the whole part in the assays by using IPTG tp stop the inhibition of LacI. To do this, pLac promoter with RBS (e.g. J04500) must be assembled.
Source
LasI sequence is gathered from Registry.