Difference between revisions of "Part:BBa K772005"

Latest revision as of 11:09, 29 September 2012

AHL generator: LasI

Quorum sensing is the mechanism of communication between bacteria via synthesizing and detecting chemical compounds in the media. With these mediators, the promoters and their yield can be tuned or regulated. This part represents an alternative quorum sensing sender device which is designed to induce PoPS production in the parts including pLas such as BBa_K772004[1]. LasI is an enzyme generator gene sequences which synthesizes 3OC12HSL (AHL) in order to induce a PoPS response in the pLas promoter. pLas promoter works in the presence of both 3OC12HSL and LasR protein.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 1581
1000
COMPATIBLE WITH RFC[1000]

Revision as of 11:09, 29 September 2012 (view source) Mustafa (Talk \| contribs) ← Older edit		Latest revision as of 11:09, 29 September 2012 (view source) Mustafa (Talk \| contribs)
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	Quorum sensing is the mechanism of communication between bacteria via synthesizing and detecting chemical compounds in the media. With these mediators, the promoters and their yield can be tuned or regulated.		Quorum sensing is the mechanism of communication between bacteria via synthesizing and detecting chemical compounds in the media. With these mediators, the promoters and their yield can be tuned or regulated.
−	This part represents an alternative quorum sensing sender device which is designed to induce PoPS production in the parts including pLas such as BBa_K772004[https://parts.igem.org/wiki/index.php?title=Part:BBa_K772004]. LasI is an enzyme generator gene sequences which synthesizes ~~3OC6HSL~~ (AHL) in order to induce a PoPS response in the pLas promoter. pLas promoter works in the presence of both ~~3OC6HSL~~ and LasR protein.	+	This part represents an alternative quorum sensing sender device which is designed to induce PoPS production in the parts including pLas such as BBa_K772004[https://parts.igem.org/wiki/index.php?title=Part:BBa_K772004]. LasI is an enzyme generator gene sequences which synthesizes 3OC12HSL (AHL) in order to induce a PoPS response in the pLas promoter. pLas promoter works in the presence of both 3OC12HSL and LasR protein.