Difference between revisions of "Part:BBa K771001"
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==Design== | ==Design== | ||
− | [[Image:12SJTU_MA1.jpg| | + | [[Image:12SJTU_MA1.jpg|100px|thumb|center]] |
− | RNA aptamer binding protein PP7([https://parts.igem.org/wiki/index.php?title=Part:BBa_K771111 BBa_K771111]) fused with membrane protein Lgt | + | RNA aptamer binding protein PP7([https://parts.igem.org/wiki/index.php?title=Part:BBa_K771111 BBa_K771111]) fused with membrane protein Lgt ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K771102 BBa_K771102]). When RNA molecule D0 is present, Membrane Anchor 1 is expected to dimerize with Membrane Anchor 2([https://parts.igem.org/wiki/index.php?title=Part:BBa_K771002 BBa_K771002]) through MS2([https://parts.igem.org/wiki/index.php?title=Part:BBa_K771112 BBa_K771112]) and PP7([https://parts.igem.org/wiki/index.php?title=Part:BBa_K771111 BBa_K771111]) domain. |
− | + | ||
− | ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K771102 BBa_K771102]). When RNA molecule D0 is present, Membrane Anchor 1 is expected to dimerize with Membrane Anchor 2([https://parts.igem.org/wiki/index.php?title=Part:BBa_K771002 BBa_K771002]) through MS2([https://parts.igem.org/wiki/index.php? | + | |
− | + | ||
− | title=Part:BBa_K771112 BBa_K771112]) and PP7([https://parts.igem.org/wiki/index.php?title=Part:BBa_K771111 BBa_K771111]) domain. | + | |
==Related Biobrick== | ==Related Biobrick== |
Revision as of 04:20, 29 September 2012
Membrane Anchor 1 :ssDsbA-Lgt-PP7
Membrane anchor 1:RNA aptamer binding protein PP7 (BBa_K771111) fused with membrane protein
Lgt(BBa_K771102).
Overview: Membrane Scaffold System
Demonstration of the whole Membrane Scaffold device. Membrane Anchor 2, 3, 4, 5 consitutively aggregate while Membrane Anchor 1 and 6 only aggregate
with constitutive cluster (Membrane Anchor 2, 3, 4, 5) when certain signal is present.
Backgroud
Membrane Anchor 1 and 2 act as a controlling point to determine whether certain pathway (in this case involving enzyme 1, 2, 3, 4 and 5) is activated or
not. RNA molecule D0(BBa_K771009) with PP7 and MS2 aptamer domain acts as the signal.
Design
RNA aptamer binding protein PP7(BBa_K771111) fused with membrane protein Lgt (BBa_K771102). When RNA molecule D0 is present, Membrane Anchor 1 is expected to dimerize with Membrane Anchor 2(BBa_K771002) through MS2(BBa_K771112) and PP7(BBa_K771111) domain.
Related Biobrick
<br\>Membrabe Anchor 2([BBa_K771002]) <br\>Membrabe Anchor 3([BBa_K771003]) <br\>Membrabe Anchor 4([BBa_K771004]) <br\>Membrabe Anchor 5([BBa_K771005]) <br\>Membrabe Anchor 6([BBa_K771006])
Reference
Delebecque, C. J., A. B. Lindner, et al. (2011). "Organization of intracellular reactions with rationally designed RNA assemblies." Science 333(6041): 470.
Reference
Delebecque, C. J., A. B. Lindner, et al. (2011). "Organization of intracellular reactions with rationally designed RNA assemblies." Science 333(6041): 470.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1129
Illegal BglII site found at 1385 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 883
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 504