Difference between revisions of "Part:BBa K771107"
 
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===Usage and Biology===
 
===Usage and Biology===
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[[Image:12SJTU_2PDZ.gif|right]]
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[[Image:12SJTU SH3dl.gif|right]]
  
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The PDZ domain along with its ligand([https://parts.igem.org/Part:BBa_K771104 Part:BBa_K771104]) comes from ''Mus musculus'', which could dimerize spontaneously. The original dimer was introduced by Dueber et al., 2012. This pair of dimer is a perfect material to construct the dimerizable proteins. In Membrane Magic system designed by [http://2012.igem.org/Team:SJTU-BioX-Shanghai 2012 SJTU-BioX-Shanghai Team], the PDZ domain is located in Membrane Anchor 4, while the PDZ ligands on Membrane Anchor 3. Due to the fact that the PDZ domain and ligand is in the N-terminus of the whole protein, we added a SRP-dependent signal peptide, ssdsb, to help the Membrane Anchor localize to the membrane correctly.
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The SH3 domain along with its ligand([https://parts.igem.org/Part:BBa_K771108 Part:BBa_K771108]) comes from ''Mus musculus'', which could dimerize spontaneously. The original dimer was introduced by Dueber et al., 2012. This pair of dimer is a perfect material to construct the dimerizable proteins. In Membrane Magic system designed by [http://2012.igem.org/Team:SJTU-BioX-Shanghai 2012 SJTU-BioX-Shanghai Team], the SH3 Domain is located in Membrane Anchor 2, while the SH3 ligand on Membrane Anchor 3. A flexible linker3(FL3) is added to the N-terminus of the domain, enabling its proper interaction with its domain on Membrane Anchor 3.
  
 
===Related Parts===
 
===Related Parts===
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[https://parts.igem.org/Part:BBa_K771104 Part:BBa_K771104, PDZ ligand]
 
  
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[https://parts.igem.org/Part:BBa_K771003 Part:BBa_K771003, Membrane Anchor 3, containing PDZ ligand]
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[https://parts.igem.org/Part:BBa_K771106 Part:BBa_K771108, SH3 ligand]
 +
 
 +
 
 +
[https://parts.igem.org/Part:BBa_K771004 Part:BBa_K771002, Membrane Anchor 2, containing SH3 domain]
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 +
 
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[https://parts.igem.org/Part:BBa_K771005 Part:BBa_K771003, Membrane Anchor 3, containing SH3 ligand]
  
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[https://parts.igem.org/Part:BBa_K771004 Part:BBa_K771004, Membrane Anchor 4, containing PDZ domaim]
 
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K771107 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K771107 SequenceAndFeatures</partinfo>

Latest revision as of 04:03, 29 September 2012

SH3domain

Components of interacting proteins

Usage and Biology

12SJTU SH3dl.gif

The SH3 domain along with its ligand(Part:BBa_K771108) comes from Mus musculus, which could dimerize spontaneously. The original dimer was introduced by Dueber et al., 2012. This pair of dimer is a perfect material to construct the dimerizable proteins. In Membrane Magic system designed by [http://2012.igem.org/Team:SJTU-BioX-Shanghai 2012 SJTU-BioX-Shanghai Team], the SH3 Domain is located in Membrane Anchor 2, while the SH3 ligand on Membrane Anchor 3. A flexible linker3(FL3) is added to the N-terminus of the domain, enabling its proper interaction with its domain on Membrane Anchor 3.

Related Parts

Part:BBa_K771108, SH3 ligand


Part:BBa_K771002, Membrane Anchor 2, containing SH3 domain


Part:BBa_K771003, Membrane Anchor 3, containing SH3 ligand

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 145