Difference between revisions of "Part:BBa K771106"

Revision as of 03:42, 29 September 2012

GBDligand

components of interacting proteins

Usage and Biology

The GBD ligand along with its domain(Part:BBa_K771105) comes from Mus musculus, which could dimerize spontaneously. The original dimer was introduced by Dueber et al., 2012. This pair of dimer is a perfect material to construct the dimerizable proteins. In Membrane Magic system designed by [http://2012.igem.org/Team:SJTU-BioX-Shanghai 2012 SJTU-BioX-Shanghai Team], the GBD ligand is located in Membrane Anchor 5, while the GBD domain on Membrane Anchor 4. A flexible linker3(FL3) is added to the N-terminus of the domain, enabling its proper interaction with its domain on Membrane Anchor 4.

Related Parts

Part:BBa_K771104, PDZ ligand

Part:BBa_K771003, Membrane Anchor 3, containing PDZ ligand

Part:BBa_K771004, Membrane Anchor 4, containing PDZ domaim Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 5: / Line 5: @@
 ===Usage and Biology===
-[[Image:12SJTU_2PDZ.gif|right]]
+[[Image:12SJTU GBDdl.gif|right]]
-The PDZ domain along with its ligand([https://parts.igem.org/Part:BBa_K771104 Part:BBa_K771104]) comes from ''Mus musculus'', which could dimerize spontaneously. The original dimer was introduced by Dueber et al., 2012. This pair of dimer is a perfect material to construct the dimerizable proteins. In Membrane Magic system designed by [http://2012.igem.org/Team:SJTU-BioX-Shanghai 2012 SJTU-BioX-Shanghai Team], the PDZ domain is located in Membrane Anchor 4, while the PDZ ligands on Membrane Anchor 3. Due to the fact that the PDZ domain and ligand is in the N-terminus of the whole protein, we added a SRP-dependent signal peptide, ssdsb, to help the Membrane Anchor localize to the membrane correctly.
+The GBD ligand along with its domain([https://parts.igem.org/Part:BBa_K771105 Part:BBa_K771105]) comes from ''Mus musculus'', which could dimerize spontaneously. The original dimer was introduced by Dueber et al., 2012. This pair of dimer is a perfect material to construct the dimerizable proteins. In Membrane Magic system designed by [http://2012.igem.org/Team:SJTU-BioX-Shanghai 2012 SJTU-BioX-Shanghai Team], the GBD ligand is located in Membrane Anchor 5, while the GBD domain on Membrane Anchor 4. A flexible linker3(FL3) is added to the N-terminus of the domain, enabling its proper interaction with its domain on Membrane Anchor 4.
 ===Related Parts===