Difference between revisions of "Part:BBa K942007:Design"

 
(→‎Design Notes)
 
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K942007 short</partinfo>
 
<partinfo>BBa_K942007 short</partinfo>
Line 7: Line 6:
  
 
===Design Notes===
 
===Design Notes===
−
The SS at the N-terminal of this protein is useful for extracellular secretion. Its purification can be done by affinity to metallic ions with 6x His tag. Api m 6 codon optimization is done to be better expressed in yeasts.
+
The Signal Sequence at the N-terminal of this protein is useful for extracellular secretion. Its purification can be done by affinity to metallic ions with 6x His tag at the C-Terminal. Api m 6 codon optimization is done to be better expressed in yeasts.
−
 
+
−
 
+
  
 
===Source===
 
===Source===

Latest revision as of 21:32, 28 September 2012

SS+Api m 6+His


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The Signal Sequence at the N-terminal of this protein is useful for extracellular secretion. Its purification can be done by affinity to metallic ions with 6x His tag at the C-Terminal. Api m 6 codon optimization is done to be better expressed in yeasts.

Source

The Api m 6 allergen native from Apis miellifera, is an allergen from the bee venom.

References