Difference between revisions of "Part:BBa K942004:Experience"
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| + | This protein was expressed in Pichia pastoris. Thanks to its B.P.S. it was also planned to be expressed in Escherichia coli. We succesfully transformed the gene into our cloning strains, but curiously, our expression strains (DE3 strains) were not even able to be transformed, except from Rosettagami DE3 pLys. We believe that the fact that Rosettagami had pLys to prevent leak expression and the other expression strains did not, leads us to the idea that there might be posibility of Der f 2 having antibacterial effects, as it is describe on the main page of this same part. | ||
| + | We were able to se the expression of this protein on a SDS-PAGE after purifying it with a His-tag affinity column. Currently, we are trying different conditions to achieve the expression in BL21 Star. | ||
Revision as of 20:37, 28 September 2012
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K942004
This protein was expressed in Pichia pastoris. Thanks to its B.P.S. it was also planned to be expressed in Escherichia coli. We succesfully transformed the gene into our cloning strains, but curiously, our expression strains (DE3 strains) were not even able to be transformed, except from Rosettagami DE3 pLys. We believe that the fact that Rosettagami had pLys to prevent leak expression and the other expression strains did not, leads us to the idea that there might be posibility of Der f 2 having antibacterial effects, as it is describe on the main page of this same part.
We were able to se the expression of this protein on a SDS-PAGE after purifying it with a His-tag affinity column. Currently, we are trying different conditions to achieve the expression in BL21 Star.
User Reviews
UNIQdd87c278565573aa-partinfo-00000000-QINU UNIQdd87c278565573aa-partinfo-00000001-QINU
This protein was expressed in Pichia pastoris. Thanks to its B.P.S. it was also planned to be expressed in Escherichia coli. We succesfully transformed the gene into our cloning strains, but curiously, our expression strains (DE3 strains) were not even able to be transformed, except from Rosettagami DE3 pLys. We believe that the fact that Rosettagami had pLys to prevent leak expression and the other expression strains did not, leads us to the idea that there might be posibility of Der f 2 having antibacterial effects, as it is describe on the main page of this same part.
We were able to se the expression of this protein on a SDS-PAGE after purifying it with a His-tag affinity column. Currently, we are trying different conditions to achieve the expression in BL21 Star.