Difference between revisions of "Part:BBa K729003"
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| − | Stand alone csgBAEFG operon for curli synthesis. Adapted from Lyon 2011. | + | Stand alone csgBAEFG operon for curli synthesis. Adapted from Lyon 2011. User experience uploaded on the original page here: https://parts.igem.org/Part:BBa_K540000:Experience#User_Reviews. |
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Revision as of 19:40, 28 September 2012
csgBAEFG operon
Stand alone csgBAEFG operon for curli synthesis. Adapted from Lyon 2011. User experience uploaded on the original page here: https://parts.igem.org/Part:BBa_K540000:Experience#User_Reviews.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1683
- 1000COMPATIBLE WITH RFC[1000]
Characterisation
Congo red binding assays were preformed to determine the presence of curli fibers.
Variations
Two additional variations on the original biobrick were designed. These include a T7 and constitutive promoter.