Difference between revisions of "Part:BBa K772002"

Latest revision as of 14:48, 28 September 2012

EpCAM Binding Dock: N terminus

This gene sequence includes an EpCAM (Epithelial p? Cell Adhesion Molecule) antigen binding domain, OmpA transmembrane protein (dock) and TEV protease N terminus. It is planned to use this subpart with BBa_772, which contains only TEV protease C terminus differently instead of N terminus, in order to induce a signal reaction in the case of EpCAM presence. EpCAM is an natural antigen which is found high significantly in the cases of some breast and colorectal cancer subtypes. On the upstream region of protein sequence, there are a constitutive pLac promoter and appropiate RBS.

To see how this part works: [1]

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 13
Illegal NheI site found at 36
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 447

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K772002 short</partinfo>
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 This gene sequence includes an EpCAM (Epithelial p? Cell Adhesion Molecule) antigen binding domain, OmpA transmembrane protein (dock) and TEV protease N terminus. It is planned to use this subpart with BBa_772, which contains only TEV protease C terminus differently instead of N terminus, in order to induce a signal reaction in the case of EpCAM presence.
 EpCAM is an natural antigen which is found high significantly in the cases of some breast and colorectal cancer subtypes.
-On the upstream region of protein sequence, there are a constitutive pLac promoter and appropiate RBS. To see how this part works: Experience.
+On the upstream region of protein sequence, there are a constitutive pLac promoter and appropiate RBS.
+To see how this part works: [https://parts.igem.org/Part:BBa%20K772002:Experience]