Difference between revisions of "Part:BBa K771005"

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<partinfo>BBa_K771005 short</partinfo>
 
<partinfo>BBa_K771005 short</partinfo>
  
Membrane anchor 5, which consists of interacting light sensor VVD ,PDZ ligand and membrane protein Lgt. Membrane Anchor 5 is proved to constitutively dimerize with Membrane Anchor 4 [[https://parts.igem.org/wiki/index.php?title=Part:BBa_K771004 BBa_K771004]]. and 6 [[https://parts.igem.org/wiki/index.php?title=Part:BBa_K771006 BBa_K771006]].
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Membrane anchor 5, which consists of interacting light sensor VVD ,PDZ ligand and membrane protein Lgt. Membrane Anchor 5 is proved to constitutively dimerize with Membrane Anchor 4 [[https://parts.igem.org/wiki/index.php?title=Part:BBa_K771004 BBa_K771004]]. and could only aggregate with Membrane Anchor 6 [[https://parts.igem.org/wiki/index.php?title=Part:BBa_K771006 BBa_K771006]] when blue light is present.
  
 
==Membrane Rudder System==
 
==Membrane Rudder System==
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==light sensor==
 
==light sensor==
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VVD protein is a blue light induced dimer  ([[https://parts.igem.org/wiki/index.php?title=Part:BBa_K771109 BBa_K771109]])
  
 
===Related Biobrick===
 
===Related Biobrick===

Revision as of 14:01, 28 September 2012

Membrane Anchor 5:ssDsbA-VVD-LGT-GBD Ligand

Membrane anchor 5, which consists of interacting light sensor VVD ,PDZ ligand and membrane protein Lgt. Membrane Anchor 5 is proved to constitutively dimerize with Membrane Anchor 4 [BBa_K771004]. and could only aggregate with Membrane Anchor 6 [BBa_K771006] when blue light is present.

Membrane Rudder System

12SJTU-MA1-MA6.jpg

Demonstration of the whole Membrane Rudder device.

light sensor

VVD protein is a blue light induced dimer ([BBa_K771109])

Related Biobrick

<br\>Membrabe Anchor 1([BBa_K771001]) <br\>Membrabe Anchor 2([BBa_K771002]) <br\>Membrabe Anchor 3([BBa_K771003]) <br\>Membrabe Anchor 4([BBa_K771004]) <br\>Membrabe Anchor 6([BBa_K771006])


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 494
    Illegal AgeI site found at 1339
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 960