Difference between revisions of "Part:BBa K771004"
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Membrane anchor 4, which consists of interacting PDZ domain and membrane protein Lgt. Membrane Anchor 4 is proved to constitutively dimerize with Membrane Anchor 3 and 5 [[https://parts.igem.org/wiki/index.php?title=Part:BBa_K771002 BBa_K771002]]. | Membrane anchor 4, which consists of interacting PDZ domain and membrane protein Lgt. Membrane Anchor 4 is proved to constitutively dimerize with Membrane Anchor 3 and 5 [[https://parts.igem.org/wiki/index.php?title=Part:BBa_K771002 BBa_K771002]]. | ||
Revision as of 13:31, 28 September 2012
Membrane Anchor 4 : ssDsbA-PDZ Domain-LGT-GBD Domain
Membrane anchor 4, which consists of interacting PDZ domain and membrane protein Lgt. Membrane Anchor 4 is proved to constitutively dimerize with Membrane Anchor 3 and 5 [BBa_K771002].
Membrane Accelerator System
All four membrane proteins are assembled together through interacting domains and ligands. Downstream enzymes are thus assembled.
It has been proved to work as expected by recruiting enzymes involved in Fatty Acid biosynthetic pathway.
Membrane Rudder System
Demonstration of the whole Membrane Rudder device.
Related Biobrick
<br\>Membrabe Anchor 1([BBa_K771001]) <br\>Membrabe Anchor 2([BBa_K771002]) <br\>Membrabe Anchor 3([BBa_K771003]) <br\>Membrabe Anchor 5([BBa_K771005]) <br\>Membrabe Anchor 6([BBa_K771006])
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 115
Illegal AgeI site found at 1174 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 345
Illegal BsaI site found at 795