Difference between revisions of "Part:BBa K771103"

Revision as of 06:35, 28 September 2012

ssDsbA-PDZdomain

Component of interacting proteins of Membrane Anchor/Rudder system.

Usage and Biology

The PDZ domain along with its ligand(Part:BBa_K771104) comes from Mus musculus, which could dimerize spontaneously. The original dimer was introduced by Dueber et al., 2012. This pair of dimer is a perfect material to construct the dimerizable proteins. In Membrane Magic system designed by [http://2012.igem.org/Team:SJTU-BioX-Shanghai 2012 SJTU-BioX-Shanghai Team], the PDZ domain is located in Membrane Anchor 4, while the PDZ ligands on Membrane Anchor 3. Due to the fact that the PDZ domain and ligand is in the N-terminus of the whole protein, we added a SRP-dependent signal peptide, ssdsb, to help the Membrane Anchor localize to the membrane correctly.

Related Parts

Part:BBa_K771104, PDZ ligand

Part:BBa_K771003, Membrane Anchor 3, containing PDZ ligand

Part:BBa_K771004, Membrane Anchor 4, containing PDZ domaim

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 115
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 345

@@ Line 12: / Line 12: @@
 ===Related Parts===
 [https://parts.igem.org/Part:BBa_K771104 Part:BBa_K771104, PDZ ligand]
 [https://parts.igem.org/Part:BBa_K771003 Part:BBa_K771003, Membrane Anchor 3, containing PDZ ligand]
 [https://parts.igem.org/Part:BBa_K771004 Part:BBa_K771004, Membrane Anchor 4, containing PDZ domaim]
 <span class='h3bb'>Sequence and Features</span>
 <partinfo>BBa_K771103 SequenceAndFeatures</partinfo>