Difference between revisions of "Part:BBa K771102"
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Inner membrane protein of ''E.coli'' | Inner membrane protein of ''E.coli'' | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
To avoid toxicity caused by foreign membrane proteins, we chose phosphatidylglycerol:: prolipoprotein diacylglyceryl transferase (Lgt) as transmembrane domain in the project. Lgt is an inner membrane protein of E.coli with seven transmembrane segments and has been successfully over expressed in E. coli without causing harm to cells. | To avoid toxicity caused by foreign membrane proteins, we chose phosphatidylglycerol:: prolipoprotein diacylglyceryl transferase (Lgt) as transmembrane domain in the project. Lgt is an inner membrane protein of E.coli with seven transmembrane segments and has been successfully over expressed in E. coli without causing harm to cells. | ||
To provide continence to the upperstream protein fusion, a flexible linker is added to the upperstream of lgt. | To provide continence to the upperstream protein fusion, a flexible linker is added to the upperstream of lgt. | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K771102 SequenceAndFeatures</partinfo> | <partinfo>BBa_K771102 SequenceAndFeatures</partinfo> | ||
Latest revision as of 06:09, 28 September 2012
Lgt with Linker FL3
Inner membrane protein of E.coli
Usage and Biology
To avoid toxicity caused by foreign membrane proteins, we chose phosphatidylglycerol:: prolipoprotein diacylglyceryl transferase (Lgt) as transmembrane domain in the project. Lgt is an inner membrane protein of E.coli with seven transmembrane segments and has been successfully over expressed in E. coli without causing harm to cells. To provide continence to the upperstream protein fusion, a flexible linker is added to the upperstream of lgt.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 805
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 426