Difference between revisions of "Part:BBa K771101"

Latest revision as of 06:01, 28 September 2012

ssDsbA-Bla

signal recognition particle (SRP)-dependent signaling sequence added before β-Lactamase ,which makes hosts become resistant to Ampicillin

Usage and Biology

This part is basically composed of two parts, ssdsbA, and β-Lactamase. The lactamase DNA was PCR cloned from the vector pETDuet-1, which contains Amplicilin Resistance. the ssdsbA was directly added to the front of lactamase using PCR primer. the ssdsbA can be recognized by signal recognition particle, and the whole protein could be transferred out to the periplasm, endowing the bacteria resistance to Amplicillin. For more information, please visit [http://2012.igem.org/Team:SJTU-BioX-Shanghai 2012 SJTU-BioX-Shanghai Wiki.]

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 794

@@ Line 6: / Line 6: @@
 ===Usage and Biology===
 This part is basically composed of two parts, ssdsbA, and &#946;-Lactamase. The lactamase DNA was PCR cloned from the vector pETDuet-1, which contains Amplicilin Resistance. the ssdsbA was directly added to the front of lactamase using PCR primer.
-the ssdsbA can be recognized by signal recognition particle, and the whole protein could be transferred out to the periplasm, endowing the bacteria resistance to Amplicillin.
+the ssdsbA can be recognized by signal recognition particle, and the whole protein could be transferred out to the periplasm, endowing the bacteria resistance to Amplicillin. For more information, please visit [http://2012.igem.org/Team:SJTU-BioX-Shanghai 2012 SJTU-BioX-Shanghai Wiki.]
+===Related Parts===
+[https://parts.igem.org/wiki/index.php?title=Part:BBa_K771401 Membrane localization detection system, BBa_K771401]
 <span class='h3bb'>Sequence and Features</span>