Difference between revisions of "Part:BBa K771007"

 
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__NOTOC__
 
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<partinfo>BBa_K771007 short</partinfo>
 
<partinfo>BBa_K771007 short</partinfo>
  
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Membrane anchor 1, which consists of interacting domain and membrane protein Lgt
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Membrane anchor 3, which consists of interacting domain and membrane protein Lgt.
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BBa_K771302:FabG from E.coli.
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===Fatty acid Biosynthetic Pathway===
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FabG involves in the biosynthesis of fatty acids in ''E.coli''. The biosynthesis pathway is shown as below:
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[[Image:12SJTU_Fatty_acid_Biosynthetic_Pathway_s.jpg|thumb|500px|center|Fatty acid biosynthesis pathways in ''E.coli'', showing the role of FabG, FabZ, FabI and TesA.]]
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The E. coli fatty acid synthesis is initiated when holo-ACP, NADPH and NADH, acetyl-CoA and malonyl-CoA undergo condensation and subsequent reduction to form butyryl-ACP. These reactions are catalyzed by the malonyl-CoA:ACP transacylase FabD, the ketosynthase FabH, the NADPH-dependent ketoreductase FabG, either the dual-function dehydratase/isomerase FabA or the monofunctional dehydratase FabZ, and the NADH-dependent enoyl reductase FabI. Then the butyryl-ACP is extended via 5-7 rounds of analogous reactions to produce a C14 to C18-ACP either fully saturated or monounsaturated. These extension cycles are catalyzed by either the ketosynthase FabB or FabF in collaboration with FabD, FabG, FabA or FabZ, and FabI. Finally, the full-length fatty acid is released from the corresponding fatty acyl-ACP via hydrolysis by C16-specific thioesterase,TesA.
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 05:32, 28 September 2012

Membrane Anchor 2 without MS2:ssDsbA-LGT-SH3 Domain

Membrane anchor 3, which consists of interacting domain and membrane protein Lgt. BBa_K771302:FabG from E.coli.

Fatty acid Biosynthetic Pathway

FabG involves in the biosynthesis of fatty acids in E.coli. The biosynthesis pathway is shown as below:

Fatty acid biosynthesis pathways in E.coli, showing the role of FabG, FabZ, FabI and TesA.

The E. coli fatty acid synthesis is initiated when holo-ACP, NADPH and NADH, acetyl-CoA and malonyl-CoA undergo condensation and subsequent reduction to form butyryl-ACP. These reactions are catalyzed by the malonyl-CoA:ACP transacylase FabD, the ketosynthase FabH, the NADPH-dependent ketoreductase FabG, either the dual-function dehydratase/isomerase FabA or the monofunctional dehydratase FabZ, and the NADH-dependent enoyl reductase FabI. Then the butyryl-ACP is extended via 5-7 rounds of analogous reactions to produce a C14 to C18-ACP either fully saturated or monounsaturated. These extension cycles are catalyzed by either the ketosynthase FabB or FabF in collaboration with FabD, FabG, FabA or FabZ, and FabI. Finally, the full-length fatty acid is released from the corresponding fatty acyl-ACP via hydrolysis by C16-specific thioesterase,TesA.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 883
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 504
    Illegal SapI.rc site found at 1150