Difference between revisions of "Part:BBa K771001"

(RNA Rudder)
(membrane anchor rudder system)
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Membrane anchor 1:RNA aptamer binding protein PP7 fused with membrane protein Lgt.  And it is a part of membrane anchor rudder system.
 
Membrane anchor 1:RNA aptamer binding protein PP7 fused with membrane protein Lgt.  And it is a part of membrane anchor rudder system.
==membrane anchor rudder system==
+
==Membrane Anchor Rudder System==
 
[[Image:12SJTU-MA1-MA6.png|500px|center]]
 
[[Image:12SJTU-MA1-MA6.png|500px|center]]
  

Revision as of 03:00, 28 September 2012

Membrane Anchor 1 :ssDsbA-Lgt-PP7

Membrane anchor 1:RNA aptamer binding protein PP7 fused with membrane protein Lgt. And it is a part of membrane anchor rudder system.

Membrane Anchor Rudder System

12SJTU-MA1-MA6.png

RNA Rudder

12SJTU RNASIGNAL.jpg

A: Sketch of signal RNA molecule (called D0) which consists of PP7 and MS2 aptamer domains. B: Through fusing PP7 and MS2 protein to our membrane device, RNA molecule in Figure A can function as a bridge to connect different proteins, thus decreasing distance between corresponding proteins.

Light Rudder

Related Biobrick

<br\>Membrabe Anchor 2([BBa_K771002]) <br\>Membrabe Anchor 3([BBa_K771003]) <br\>Membrabe Anchor 4([BBa_K771004]) <br\>Membrabe Anchor 5([BBa_K771005]) <br\>Membrabe Anchor 6([BBa_K771006])

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1129
    Illegal BglII site found at 1385
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 883
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 504