Difference between revisions of "Part:BBa K771001"
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<partinfo>BBa_K771001 short</partinfo> | <partinfo>BBa_K771001 short</partinfo> | ||
− | RNA aptamer binding protein PP7 fused with membrane protein Lgt | + | Membrane anchor 1:RNA aptamer binding protein PP7 fused with membrane protein Lgt. And it is a part of membrane anchor rudder system. |
+ | ==membrane anchor rudder system== | ||
+ | [[Image:12SJTU-MA1-MA6.png]] | ||
+ | |||
+ | ==RNA Rudder== | ||
[[Image:12SJTU RNASIGNAL.jpg|500px]] | [[Image:12SJTU RNASIGNAL.jpg|500px]] | ||
A: Sketch of signal RNA molecule (called D0) which consists of PP7 and MS2 aptamer domains. B: Through fusing PP7 and MS2 protein to our membrane device, RNA molecule in Figure A can function as a bridge to connect different proteins, thus decreasing distance between corresponding proteins. | A: Sketch of signal RNA molecule (called D0) which consists of PP7 and MS2 aptamer domains. B: Through fusing PP7 and MS2 protein to our membrane device, RNA molecule in Figure A can function as a bridge to connect different proteins, thus decreasing distance between corresponding proteins. | ||
+ | ==Light Rudder== | ||
Related Biobrick: | Related Biobrick: |
Revision as of 02:56, 28 September 2012
Membrane Anchor 1 :ssDsbA-Lgt-PP7
Membrane anchor 1:RNA aptamer binding protein PP7 fused with membrane protein Lgt. And it is a part of membrane anchor rudder system.
membrane anchor rudder system
RNA Rudder
A: Sketch of signal RNA molecule (called D0) which consists of PP7 and MS2 aptamer domains. B: Through fusing PP7 and MS2 protein to our membrane device, RNA molecule in Figure A can function as a bridge to connect different proteins, thus decreasing distance between corresponding proteins.
Light Rudder
Related Biobrick: <br\>Membrabe Anchor 2([BBa_K771002]) <br\>Membrabe Anchor 3([BBa_K771003]) <br\>Membrabe Anchor 4([BBa_K771004]) <br\>Membrabe Anchor 5([BBa_K771005]) <br\>Membrabe Anchor 6([BBa_K771006])
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1129
Illegal BglII site found at 1385 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 883
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 504