Difference between revisions of "Part:BBa K771001"

Revision as of 02:49, 28 September 2012

Membrane Anchor 1 :ssDsbA-Lgt-PP7

RNA aptamer binding protein PP7 fused with membrane protein Lgt

A: Sketch of signal RNA molecule (called D0) which consists of PP7 and MS2 aptamer domains. B: Through fusing PP7 and MS2 protein to our membrane device, RNA molecule in Figure A can function as a bridge to connect different proteins, thus decreasing distance between corresponding proteins.

Related Biobrick: <br\>Membrabe Anchor 2([BBa_K771002]) <br\>Membrabe Anchor 3([BBa_K771003]) <br\>Membrabe Anchor 4([BBa_K771004]) <br\>Membrabe Anchor 5([BBa_K771005]) <br\>Membrabe Anchor 6([BBa_K771006])

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 1129
Illegal BglII site found at 1385
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 883
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 504

@@ Line 3: / Line 3: @@
 RNA aptamer binding protein PP7 fused with membrane protein Lgt
-[[Image:12SJTU-MA1-MA6.png|500px|center]]
+[[Image:12SJTU RNASIGNAL.jpg|500px]]
+A: Sketch of signal RNA molecule (called D0) which consists of PP7 and MS2 aptamer domains. B: Through fusing PP7 and MS2 protein to our membrane device, RNA molecule in Figure A can function as a bridge to connect different proteins, thus decreasing distance between corresponding proteins.
-As there is no compartment in prokaryotic cells, enzymes involved in a biochemical pathway diffuse all over the cytoplasm. Intermediates produced from one enzyme cannot be passed efficiently to the next due to spatial obstacles.
-Rationally designed assemblies of enzymes could help substrates flow by decreasing distance that intermediates have to travel between enzymes, and thus increase yields of sequential biological reactions. If we attach those enzymes to engineered membrane proteins which constitutively assemble together, the reactions are going to proceed much faster.
 Related Biobrick: