Difference between revisions of "Part:BBa K778002"

Revision as of 15:34, 27 September 2012

fhlA-E363G (engineered trascriptional activator fhlA with a mutation)

FhlA is the trnascriptional activator of hycAp Part:BBa K778000 .E.coli overexpressing FhlA produce H2 (since hycAp is the promoter of FHL(formate hydrogen lyase)).

fhlA-E363G has one point mutation (A to G, glutamic acid to glycine). This mutation increased H2 production under the conditions in the previous research (Viviana et.al.2009). However, in our research (team UT-Tokyo 2012[http://2012.igem.org/Team:UT-Tokyo/Project/H2_E.coli]), this mutation did not increase H2 production.

Detailed Information :[http://2012.igem.org/Team:UT-Tokyo/Project/H2_E.coli]

Sequence and Features