Difference between revisions of "Part:BBa K771305"

Revision as of 14:34, 27 September 2012

TesA with Membrane Anchor 2 (without MS2)

TesA is a cytoplasmic mutant of the periplasmic thioesterase. It is capable of releasing free fatty acids, preventing the fatty acids from being directly harnessed for phospholipid biosynthesis.

Membrane anchor 2 without MS2 is described at BBa_K771007.

TesA is linked to C terminal of Membrane anchor 2 without MS2 by Flexible Linker 3(Fl3).

Fatty acid Biosynthetic Pathway

TesA involves in the biosynthesis of fatty acids in E.coli. The biosynthesis pathway is shown as below:

The E. coli fatty acid synthesis is initiated when holo-ACP, NADPH and NADH, acetyl-CoA and malonyl-CoA undergo condensation and subsequent reduction to form butyryl-ACP. These reactions are catalyzed by the malonyl-CoA:ACP transacylase FabD, the ketosynthase FabH, the NADPH-dependent ketoreductase FabG, either the dual-function dehydratase/isomerase FabA or the monofunctional dehydratase FabZ, and the NADH-dependent enoyl reductase FabI. Then the butyryl-ACP is extended via 5-7 rounds of analogous reactions to produce a C14 to C18-ACP either fully saturated or monounsaturated. These extension cycles are catalyzed by either the ketosynthase FabB or FabF in collaboration with FabD, FabG, FabA or FabZ, and FabI. Finally, the full-length fatty acid is released from the corresponding fatty acyl-ACP via hydrolysis by C16-specific thioesterase,TesA.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 884
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 505
Illegal SapI.rc site found at 1151

@@ Line 4: / Line 4: @@
 TesA is a cytoplasmic mutant of the periplasmic thioesterase. It is capable of releasing free fatty acids, preventing the fatty acids  from being directly harnessed for phospholipid biosynthesis.
+Membrane anchor 2 without MS2 is described at BBa_K771007.
+TesA is linked to C terminal of Membrane anchor 2 without MS2 by Flexible Linker 3(Fl3).
 ===Fatty acid Biosynthetic Pathway===
-It involves in the biosynthesis of fatty acids in ''E.coli''. The biosynthesis pathway is shown as below:
+TesA involves in the biosynthesis of fatty acids in ''E.coli''. The biosynthesis pathway is shown as below:
 The ''E. coli'' fatty acid synthesis is initiated when holo-ACP, NADPH and NADH, acetyl-CoA and malonyl-CoA undergo condensation and subsequent reduction to form butyryl-ACP. These reactions are catalyzed by the malonyl-CoA:ACP transacylase FabD, the ketosynthase FabH, the NADPH-dependent ketoreductase FabG, either the dual-function dehydratase/isomerase FabA or the monofunctional dehydratase FabZ, and the NADH-dependent enoyl reductase FabI. Then the butyryl-ACP is extended via 5-7 rounds of analogous reactions to produce a C14 to C18-ACP either fully saturated or monounsaturated. These extension cycles are catalyzed by either the ketosynthase FabB or FabF in collaboration with FabD, FabG, FabA or FabZ, and FabI. Finally, the full-length fatty acid is released from the corresponding fatty acyl-ACP via hydrolysis by C16-specific thioesterase,TesA.