Difference between revisions of "Part:BBa K771305"
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==TesA with Membrane Anchor 2 (without MS2)== | ==TesA with Membrane Anchor 2 (without MS2)== | ||
| − | TesA is a cytoplasmic mutant of the periplasmic thioesterase. It is capable of releasing free fatty acids, preventing the fatty acids from being directly harnessed for phospholipid biosynthesis. It involves in the biosynthesis of fatty acids in ''E.coli''. The biosynthesis pathway is shown as below: | + | TesA is a cytoplasmic mutant of the periplasmic thioesterase. It is capable of releasing free fatty acids, preventing the fatty acids from being directly harnessed for phospholipid biosynthesis. |
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| + | <!-- Add more about the biology of this part here | ||
| + | ===Usage and Biology=== | ||
| + | It involves in the biosynthesis of fatty acids in ''E.coli''. The biosynthesis pathway is shown as below: | ||
[[File:12SJTU Fatty acid Biosynthetic Pathway s.jpg|thumb|center|650px|Fatty acid biosynthesis pathways in ''E.coli'', showing the role of FabG, FabZ, FabI and TesA.]] | [[File:12SJTU Fatty acid Biosynthetic Pathway s.jpg|thumb|center|650px|Fatty acid biosynthesis pathways in ''E.coli'', showing the role of FabG, FabZ, FabI and TesA.]] | ||
Catalytic cycle of the E. coli fatty acid synthesis is initiated when holo-ACP, NADPH and NADH, acetyl-CoA and malonyl-CoA undergo condensation and subsequent reduction to form butyryl-ACP. These reactions are catalyzed by the malonyl-CoA:ACP transacylase FabD, the ketosynthase FabH, the NADPH-dependent ketoreductase FabG, either the dual-function dehydratase/isomerase FabA or the monofunctional dehydratase FabZ, and the NADH-dependent enoyl reductase FabI. Then the butyryl-ACP is extended via 5-7 rounds of analogous reactions to produce a C14 to C18-ACP either fully saturated or monounsaturated. These extension cycles are catalyzed by either the ketosynthase FabB or FabF in collaboration with FabD, FabG, FabA or FabZ, and FabI. Finally, the full-length fatty acid is released from the corresponding fatty acyl-ACP via hydrolysis by C16-specific thioesterase,TesA or another more promising candidate, C18-specific thioesterase, FatA. | Catalytic cycle of the E. coli fatty acid synthesis is initiated when holo-ACP, NADPH and NADH, acetyl-CoA and malonyl-CoA undergo condensation and subsequent reduction to form butyryl-ACP. These reactions are catalyzed by the malonyl-CoA:ACP transacylase FabD, the ketosynthase FabH, the NADPH-dependent ketoreductase FabG, either the dual-function dehydratase/isomerase FabA or the monofunctional dehydratase FabZ, and the NADH-dependent enoyl reductase FabI. Then the butyryl-ACP is extended via 5-7 rounds of analogous reactions to produce a C14 to C18-ACP either fully saturated or monounsaturated. These extension cycles are catalyzed by either the ketosynthase FabB or FabF in collaboration with FabD, FabG, FabA or FabZ, and FabI. Finally, the full-length fatty acid is released from the corresponding fatty acyl-ACP via hydrolysis by C16-specific thioesterase,TesA or another more promising candidate, C18-specific thioesterase, FatA. | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
Revision as of 14:20, 27 September 2012
TesA with Membrane Anchor 2 (without MS2)
TesA with Membrane Anchor 2 (without MS2)
TesA is a cytoplasmic mutant of the periplasmic thioesterase. It is capable of releasing free fatty acids, preventing the fatty acids from being directly harnessed for phospholipid biosynthesis.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 884
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 505
Illegal SapI.rc site found at 1151