Difference between revisions of "Part:BBa J100094:Experience"
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===Applications of BBa_J100094=== | ===Applications of BBa_J100094=== | ||
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| + | We tested the lac promoter through the Golden Gate Assembly Method. This promoter regulates the transcription of the lac operon in E.coli. We measured this promoter's strength as a measure of fluorescence under the 7 different following conditions: a positive (plasmid, red fluorescent protein, and RBS) and two negative controls (broth and plasmid without promoter), a small culture with and without IPTG, and a large culture with and without IPTG. IPTG is a compound that triggers the transcription of the lac operon. | ||
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| + | [[Image:CHVTlac2.png]] | ||
===User Reviews=== | ===User Reviews=== | ||
Latest revision as of 13:51, 27 September 2012
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Applications of BBa_J100094
We tested the lac promoter through the Golden Gate Assembly Method. This promoter regulates the transcription of the lac operon in E.coli. We measured this promoter's strength as a measure of fluorescence under the 7 different following conditions: a positive (plasmid, red fluorescent protein, and RBS) and two negative controls (broth and plasmid without promoter), a small culture with and without IPTG, and a large culture with and without IPTG. IPTG is a compound that triggers the transcription of the lac operon.
User Reviews
UNIQ615c2e63f078ef43-partinfo-00000000-QINU UNIQ615c2e63f078ef43-partinfo-00000001-QINU