Difference between revisions of "Add a Part to the Registry"

Revision as of 14:17, 6 June 2006

Members of Registry groups may add three kinds of parts to the registry: Basic Parts, Composite Parts, and Construction Intermediates.
Just starting? Need help? Check out our documentation on How to make a Biobrick

Basic Parts Add a Basic Part Now...

Basic Parts are atomic units of DNA. They are not assembled from other parts in the Registry. The design for a basic part must specify the exact sequence of the part, provide annotation of that sequence, and design notes about the sequence itself. The DNA for a basic part may be obtained by de-novo synthesis, by primer extension and PCR, or other microbiological techniques. Like all parts, a Basic Part is stored in a plasmid, surrounded by a restriction-enzyme cloning region. This cloning region is not included in the sequence of the part. It can be provided by the Registry software.

Composite Parts Add a Composite Part Now...

Composite Parts are made from an ordered list of basic parts or other composite parts. An explicit sequence of DNA cannot be included in the list, but should first be added to the Registry as a basic part. The Registry maintains the list of subparts given by the designer as primary documentation of a composite part. The Registry software also provides a list of all the basic parts that make up the composite part. This is called the deep parts list and is used when searching for parts to use during an assembly. Composite parts should be documented in full detail. While the Registry provides the sequence and annotations from the component parts specified, the function and design issues of the composite part should be documented in detail.

Construction Intermediates Add a Construction Intermediate Now...

Construction Intermediates have no specific function and are just the result of assembling two parts together. They require no further documentation. They all have the type 'Intermediate' and part names of the form 'BBa_Snnnnn'. These part names are automatically assigned by the Registry software. Once you enter your intermediate part in the Registry, you will be able to use BioBrick Blast to check your assembly's sequence and your part will show up in the subpart and superpart search functions. If you send us the DNA, we will be able to share your work with others and include it in assemblies done by the Registry.