Difference between revisions of "Part:BBa K801081:Design"
(→‎Design Notes)
 
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'''Related BioBrick:'''
 
'''Related BioBrick:'''
 
<!--*Other versions:[https://parts.igem.org/wiki/index.php?title=Part:BBa_?????? BBa_??????: Name_of_part] -->
 
<!--*Other versions:[https://parts.igem.org/wiki/index.php?title=Part:BBa_?????? BBa_??????: Name_of_part] -->
−
<!--*Related BioBricks:[https://parts.igem.org/wiki/index.php?title=Part:BBa_?????? BBa_??????: Name_of_part]
+
* Related BioBricks:[https://parts.igem.org/wiki/index.php?title=Part:BBa_K801080 BBa_K801080: Preprothaumatin]
  
 
'''Cloning details:'''<br>
 
'''Cloning details:'''<br>
−
<!--*Designed in RFC10/RFC23/RFC25/RFC25_N-part-->
+
* Designed in RFC10
 
<!--*Mutation C889G to delete XbaI restriction site-->
 
<!--*Mutation C889G to delete XbaI restriction site-->
 
<!--*Truncation upstream/downstream compared to template, ?explanation?-->
 
<!--*Truncation upstream/downstream compared to template, ?explanation?-->
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<!--*Test digestion using ?enzyme1? & ?enzyme2?/Not yet performed-->
 
<!--*Test digestion using ?enzyme1? & ?enzyme2?/Not yet performed-->
 
<!--*Sequencing using primer ?primer_name?/Not yet sequenced-->
 
<!--*Sequencing using primer ?primer_name?/Not yet sequenced-->
−
<!--*Part was partly sequenced/Part was totally sequenced-->
+
* Part was totally sequenced
  
 
'''Backbone:'''<br>
 
'''Backbone:'''<br>
−
<!--*Backbone name: pSB1C3'/?backbone_name?-->
+
* Backbone name: pSB1C3
 
<!--*Resistance: Amp/Cp/Kan/Tet-->
 
<!--*Resistance: Amp/Cp/Kan/Tet-->
 
<!--*Copynumber: low/medium/high-->
 
<!--*Copynumber: low/medium/high-->
  
 
'''Protein coding:'''<br>
 
'''Protein coding:'''<br>
−
<!--*Protein: ?Name_of_gene_product? [Nucleotide 1 to ???]-->
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* Protein: Preprothaumatin [Nucleotide x to x]
 
<!--*The protein has the amino acid replacements ???99??? to ???99???.-->
 
<!--*The protein has the amino acid replacements ???99??? to ???99???.-->
−
<!--*The protein encoded is posttranslationally modified by ???.-->
+
* The protein encoded is posttranslationally modified: Pre- and Pro-sequence are split off
 
<!--*Tag: n-terminally fused/c-terminally fused His5/His6/Strep/Flag/other-->
 
<!--*Tag: n-terminally fused/c-terminally fused His5/His6/Strep/Flag/other-->
  
 
'''Enzymatic activity:'''
 
'''Enzymatic activity:'''
−
<!--none/EC-number ?.?.?.?-->
+
* none
  
 
'''Cytotoxicity:'''<br>
 
'''Cytotoxicity:'''<br>
−
<!--none/not known/cytotoxic for ''organism name''-->
+
* none
  
 
'''Safety notes:'''<br>
 
'''Safety notes:'''<br>
−
<!--Known and anticipated sefety issues: none/health_risk/environmental_risk/other_risk-->
+
* none
 
<!--Known and anticipated security issues: none/other.-->
 
<!--Known and anticipated security issues: none/other.-->
  

Latest revision as of 07:06, 27 September 2012

Prepro-Thaumatin expression cassette for yeast


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 205



Based on part BBa_K801080 (Prepro-Thaumatin).


Keywords:


Abbreviations:

Design Notes

Related BioBrick:

Cloning details:

  • Designed in RFC10

Quality control measures:

  • Part was totally sequenced

Backbone:

  • Backbone name: pSB1C3

Protein coding:

  • Protein: Preprothaumatin [Nucleotide x to x]
  • The protein encoded is posttranslationally modified: Pre- and Pro-sequence are split off

Enzymatic activity:

  • none

Cytotoxicity:

  • none

Safety notes:

  • none

Intellectual property:

Corresponding part author/authors:

Source

Source:


Organism:


References

Literature references:

Database references: