Difference between revisions of "Part:BBa K818400"

Revision as of 03:50, 27 September 2012

AmilCP with sboA regulator

This is a coding construct for B. subtilis to produce AmilCP pigment in response to rotten meat volatiles in the environment. AmilCP is a blue pigment designed by iGEM Uppsala 2011 and the regulator, sboA, is designed by iGEM Groningen 2012.

Usage and Biology

Expression in E. coli SboA-AmilCP is strongly expressed in E. coli, on plate and in liquid culture, at normal growth conditions. On plate, the blue-purple colour is less visible compared to the cell pellet in liquid culture.

Expression in B. subtilis

SboA-AmilGFP was shown to be very weakly expressed in Bacillus subtilis on LB plate without rotten meat induction (faint color formation after 4 days). This is probably due to the leakiness of the promoter.

We (iGEM Groningen 2012) tested the expression of SboA-AmilCP in Bacillus subtilis subjected to volatiles from spoiled meat using the same setup as we used to test the sticker design (see our sticker page). After this setup, a clear blue-purple color was visible.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 207
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 11: / Line 11: @@
 '''Expression in <i>B. subtilis</i>'''
-SboA-AmilGFP was shown to be very weakly expressed in <i>Bacillus subtilis</i> on LB plate without rotten meat induction (faint color formation after 2 days). This is probably due to the leakiness of the promoter.
+SboA-AmilGFP was shown to be very weakly expressed in <i>Bacillus subtilis</i> on LB plate without rotten meat induction (faint color formation after 4 days). This is probably due to the leakiness of the promoter.
+We (iGEM Groningen 2012) tested the expression of SboA-AmilCP in <i>Bacillus subtilis</i> subjected to volatiles from spoiled meat using the same setup as we used to test the sticker design (see our sticker page). After this setup, a clear blue-purple color was visible.
-We (iGEM Groningen 2012) tested the expression of SboA-AmilCP in <i>Bacillus subtilis</i> subjected to volatiles from spoiled meat using the same setup as we used to test the sticker design (see our sticker page).
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