Difference between revisions of "Part:BBa K861038:Experience"

(→‎Applications of BBa_K861038)
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https://static.igem.org/mediawiki/parts/b/bc/Cupric_acetate-1-.png
 
https://static.igem.org/mediawiki/parts/b/bc/Cupric_acetate-1-.png
  
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In this picture , the color of the sample  C    standing for cell extracts collected from bacteria overexpressing Samonella fadA and Samonella fadB is light than the controls . It means the fatty acids remaining in the tube is less .From the results above, we have proved that the biobrick responsible for overexpressing fadD can metabolize fatty acids much faster.
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In this picture , the color of the sample  C    standing for cell extracts collected from bacteria overexpressing Samonella fadA and Samonella fadB is light than the controls . It means the fatty acids remaining in the tube is less .From the result above, we have proved that the biobrick responsible for overexpressing the mentioned genes can metabolize fatty acids much faster.
  
 
For more details, please visit our wiki http://2012.igem.org/Team:WHU-China
 
For more details, please visit our wiki http://2012.igem.org/Team:WHU-China

Revision as of 03:06, 27 September 2012

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Applications of BBa_K861038

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iGEM2012 WHU-China

In the in vitro assay we performed, we collected cell extracts from 1L medium and got protein solution of high concentration .The enzyme assay was then performed to measure the ability of degrading fatty acids by single enzyme and their different combinations.The remaining amount of fatty acids in the medium is measured by the cupric-acetate reaction . Cupric_acetate-1-.png In this picture , the color of the sample C standing for cell extracts collected from bacteria overexpressing Samonella fadA and Samonella fadB is light than the controls . It means the fatty acids remaining in the tube is less .From the result above, we have proved that the biobrick responsible for overexpressing the mentioned genes can metabolize fatty acids much faster. For more details, please visit our wiki http://2012.igem.org/Team:WHU-China

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User Reviews

UNIQ7f8e396d39f4b7c3-partinfo-00000001-QINU

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