Difference between revisions of "Part:BBa K861024:Experience"

 
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
 
This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
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===Applications of BBa_K861024===
 
===Applications of BBa_K861024===
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{|width='80%' style='border:1px solid gray'
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<partinfo>BBa_K861002 AddReview 1</partinfo>
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<I>iGEM2012 WHU-China</I>
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<p>In the in vitro assay we performed, we collected cell extracts from 1L medium and got protein solution of high concentration .The enzyme assay was then performed to measure the ability of degrading fatty acids by single enzyme and their different combinations.The remaining amount of fatty acids in the medium is measured by the cupric-acetate reaction .
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https://static.igem.org/mediawiki/parts/b/bc/Cupric_acetate-1-.png
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In this picture , the color of the sample      standing for cell extracts collected from bacteria overexpressing fadD is light than the controls . It means the fatty acids remaining in the tube is less .From the results above, we have proved that the biobrick responsible for overexpressing fadD can metabolize fatty acids much faster.
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For more details, please visit our wiki http://2012.igem.org/Team:WHU-China
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===User Reviews===
 
===User Reviews===

Revision as of 02:57, 27 September 2012

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K861024

•

iGEM2012 WHU-China

In the in vitro assay we performed, we collected cell extracts from 1L medium and got protein solution of high concentration .The enzyme assay was then performed to measure the ability of degrading fatty acids by single enzyme and their different combinations.The remaining amount of fatty acids in the medium is measured by the cupric-acetate reaction . Cupric_acetate-1-.png In this picture , the color of the sample standing for cell extracts collected from bacteria overexpressing fadD is light than the controls . It means the fatty acids remaining in the tube is less .From the results above, we have proved that the biobrick responsible for overexpressing fadD can metabolize fatty acids much faster. For more details, please visit our wiki http://2012.igem.org/Team:WHU-China

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User Reviews

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