Difference between revisions of "Part:BBa K945001"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K945001 short</partinfo> | <partinfo>BBa_K945001 short</partinfo> | ||
− | This sequence contains the inducible transcription promoter FLD1, useful for controlled gene expression which is actively enhanced in Pichia pastoris in the presence of methylamine or methanol. | + | This sequence contains the inducible transcription promoter FLD1, useful for controlled gene expression which is actively enhanced in <i>Pichia pastoris</i> in the presence of methylamine or methanol. |
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<partinfo>BBa_K945001 SequenceAndFeatures</partinfo> | <partinfo>BBa_K945001 SequenceAndFeatures</partinfo> | ||
+ | ===Promoter Strength=== | ||
+ | Tec-Monterrey Ekam is working on further characterizing the functionality of this part through the use of GFP as a reporter gene, which will be finalized shortly. | ||
<!-- Uncomment this to enable Functional Parameter display | <!-- Uncomment this to enable Functional Parameter display |
Revision as of 02:39, 27 September 2012
FLD1 yeast promoter
This sequence contains the inducible transcription promoter FLD1, useful for controlled gene expression which is actively enhanced in Pichia pastoris in the presence of methylamine or methanol.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 25
Illegal EcoRI site found at 342
Illegal EcoRI site found at 675
Illegal XbaI site found at 797 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 25
Illegal EcoRI site found at 342
Illegal EcoRI site found at 675 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 25
Illegal EcoRI site found at 342
Illegal EcoRI site found at 675 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 25
Illegal EcoRI site found at 342
Illegal EcoRI site found at 675
Illegal XbaI site found at 797 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 25
Illegal EcoRI site found at 342
Illegal EcoRI site found at 675
Illegal XbaI site found at 797 - 1000COMPATIBLE WITH RFC[1000]
Promoter Strength
Tec-Monterrey Ekam is working on further characterizing the functionality of this part through the use of GFP as a reporter gene, which will be finalized shortly.