Difference between revisions of "Part:BBa K945000"
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This sequence contains the strong inducible transcription promoter AOX1, useful for controlled gene expression which is actively enhanced in <i>Pichia pastoris</i> in the presence of methanol.
 
This sequence contains the strong inducible transcription promoter AOX1, useful for controlled gene expression which is actively enhanced in <i>Pichia pastoris</i> in the presence of methanol.
  
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<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===
  

Revision as of 02:39, 27 September 2012

AOX1 yeast promoter

This sequence contains the strong inducible transcription promoter AOX1, useful for controlled gene expression which is actively enhanced in Pichia pastoris in the presence of methanol.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1016
    Illegal PstI site found at 47
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 47
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 53
    Illegal BglII site found at 75
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1016
    Illegal PstI site found at 47
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1016
    Illegal PstI site found at 47
    Illegal NgoMIV site found at 34
  • 1000
    COMPATIBLE WITH RFC[1000]

Promoter Strength

Tec-Monterrey Ekam is working on further characterizing the functionality of this part through the use of GFP as a reporter gene, which will be finalized shortly.