Difference between revisions of "Part:BBa K864202"
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| + | MarR(G95S) (multi antibiotic resistance repressor) is the native repressor of the E coli Mar operon. The operon consist of two parts: the MarC and the MarRAB which both is controlled by the operator MarO, which MarR represses. The Mar operon has been shown to cause resistance including Tetracycline, chloramphenicol, b-lactams, and quinolones [1]. | ||
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| + | MarR(G95S) is amplified from the E coli K-12 substrain MG1655 by nested-PCR and point mutated in G95S with PCR to get higher repression[2]. | ||
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Latest revision as of 01:59, 27 September 2012
MarR(G95S) super-repressor
MarR(G95S) (multi antibiotic resistance repressor) is the native repressor of the E coli Mar operon. The operon consist of two parts: the MarC and the MarRAB which both is controlled by the operator MarO, which MarR represses. The Mar operon has been shown to cause resistance including Tetracycline, chloramphenicol, b-lactams, and quinolones [1].
MarR(G95S) is amplified from the E coli K-12 substrain MG1655 by nested-PCR and point mutated in G95S with PCR to get higher repression[2].
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]