Difference between revisions of "Part:BBa K778008:Experience"

 
 
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===Applications of BBa_K778008===
 
===Applications of BBa_K778008===
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We measured the amount of H2 produced by E.coli transformed with either “pLac-RBS-fhlA-d.term”(fhlA) or “pLac-RBS-fhlA(E363G)-d.term”(fhlA (E363G)).
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The method is written in the following page(http://2012.igem.org/Team:UT-Tokyo/LabWork/AssayMethods).
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The result is the following (Fig.1)
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https://static.igem.org/mediawiki/2012/a/aa/UT_Tokyo_Fig_diagram_640_480.png
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Fig.1:H2production in liquid LB medium containing the labeled glucose concentration.
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The vertical axis expresses v/v% of H2 in the gaseous phase after we cultivate each E.coli for 8 hours in a container with 1mL each of LB medium and the gaseous phase .The horizontal axis shows the kinds of E.coli and the concentrations of glucose in each medium(“LB+x%” means the glucose w/v concentrations of liquid LB medium is x% more than the usual medium.
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The results shown by the Fig.1 is statistically sufficient to conclude the followings.
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*The amount of H2 production is fhlA > fhlA (E363G) > wild type in these conditions.
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*There is a positive correlation between the amount of H2 produced by fhlA and the glucose concentration in the liquid LB.
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The detailed interpretations of this result are done in this page→http://2012.igem.org/Team:UT-Tokyo/Project/H2_E.coli/Discussion.
  
 
===User Reviews===
 
===User Reviews===

Latest revision as of 01:59, 27 September 2012

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K778008

We measured the amount of H2 produced by E.coli transformed with either “pLac-RBS-fhlA-d.term”(fhlA) or “pLac-RBS-fhlA(E363G)-d.term”(fhlA (E363G)).

The method is written in the following page(http://2012.igem.org/Team:UT-Tokyo/LabWork/AssayMethods).

The result is the following (Fig.1)

UT_Tokyo_Fig_diagram_640_480.png

Fig.1:H2production in liquid LB medium containing the labeled glucose concentration. The vertical axis expresses v/v% of H2 in the gaseous phase after we cultivate each E.coli for 8 hours in a container with 1mL each of LB medium and the gaseous phase .The horizontal axis shows the kinds of E.coli and the concentrations of glucose in each medium(“LB+x%” means the glucose w/v concentrations of liquid LB medium is x% more than the usual medium.

The results shown by the Fig.1 is statistically sufficient to conclude the followings.

  • The amount of H2 production is fhlA > fhlA (E363G) > wild type in these conditions.
  • There is a positive correlation between the amount of H2 produced by fhlA and the glucose concentration in the liquid LB.

The detailed interpretations of this result are done in this page→http://2012.igem.org/Team:UT-Tokyo/Project/H2_E.coli/Discussion.

User Reviews

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