Difference between revisions of "Part:BBa K817050:Design"
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===Source=== | ===Source=== | ||
| + | "Transcription from a heat-inducible promoter causes heat shock regulation of the sigma subunit of E. coli RNA polymerase", Cell press, 1984 | ||
ATGCTGCCACCCTTGAAAAACTGTCGATGTGGGACGATATAGCAGAT(+1) | ATGCTGCCACCCTTGAAAAACTGTCGATGTGGGACGATATAGCAGAT(+1) | ||
===References=== | ===References=== | ||
Latest revision as of 01:56, 27 September 2012
Novel thermal promoter, Phs
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
In the paper, the clone the promoter upstream from transcription starting site till -85 region; however, we only clone up till -45 regions, in which -45~-35 region is proven to be critical to the promoter function.
For our functional assay result, please refer to our [http://2012.igem.org/Team:NTU-Taida/Result/Thermal-Promoter wiki page].
Source
"Transcription from a heat-inducible promoter causes heat shock regulation of the sigma subunit of E. coli RNA polymerase", Cell press, 1984 ATGCTGCCACCCTTGAAAAACTGTCGATGTGGGACGATATAGCAGAT(+1)