Difference between revisions of "Part:BBa K808010"

Revision as of 00:58, 27 September 2012

tphB: reaction from DCD to protocatechuate

Homology modelling of TphB. For simulation parameters http://2012.igem.org/Team:TU_Darmstadt/Modeling_Homologie_Modeling#TphB click here.

TphB is coding for the decarboxylating cis-dihydrodiol dehydrogenase from Comamonas testosteroni Kf-1. It catalyzes the second reaction in the terephthalic acid degration - the decarboxylation of DCD to protocatechuate. The [http://2012.igem.org/Team:TU_Darmstadt/Modeling_Homologie_Modeling#TphB homology modeling] suggests a homodimerization as described by the recent paper by Bains et al.
For further information, please visit the [http://2012.igem.org/Team:TU_Darmstadt/Labjournal/Metabolism TU Darmstadt iGEM team wiki].

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 155
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 202
Illegal NgoMIV site found at 877
Illegal AgeI site found at 939
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI site found at 76

Part Characterisation

Figure 1. Note the peak in GPC at about 32 minutes (about 64 kDa)

GPC confirmed the suggested homodimerization (Figure 1).

References

Sasoh, M., E. Masai, et al. (2006). "Characterization of the terephthalate degradation genes of Comamonas sp. strain E6." Appl Environ Microbiol 72(3): 1825-1832.
Fukuhara, Y., K. Inakazu, et al. (2010). "Characterization of the isophthalate degradation genes of Comamonas sp. strain E6." Appl Environ Microbiol 76(2): 519-527.
Kamimura, N., T. Aoyama, et al. (2010). "Characterization of the protocatechuate 4,5-cleavage pathway operon in Comamonas sp. strain E6 and discovery of a novel pathway gene." Appl Environ Microbiol 76(24): 8093-8101.
Schläfli HR, Weiss MA, Leisinger T, Cook AM. (1994)"Terephthalate 1,2-dioxygenase system from Comamonas testosteroni T-2: purification and some properties of the oxygenase component." J Bacteriol. 1994 Nov;176(21):6644-52.
Bains, J., J.E. Wulff, and M.J. Boulanger, Investigating Terephthalate Biodegradation: Structural Characterization of a Putative Decarboxylating cis-Dihydrodiol Dehydrogenase. J Mol Biol, 2012.

@@ Line 38: / Line 38: @@
 ==Part Characterisation==
-[[Image:TphB.JPG|300px|thumb|right| Figure 1. Note the peak in FPLC at about 32 minutes (about 64 kDa)]]
+[[Image:TphB.JPG|300px|thumb|right| Figure 1. Note the peak in GPC at about 32 minutes (about 64 kDa)]]
-FPLC confirmed the suggested homodimerization (Figure 1).
+GPC confirmed the suggested homodimerization (Figure 1).
 ==References==