Difference between revisions of "Part:BBa K899006"

Revision as of 00:51, 27 September 2012

heat inducible FFT with TA system compatible with BBa_K899005

Heat inducible promoter K33801 regulating the expression of fructan:fructan fructosyltransferase (FFT) gene and a toxin-antitoxin (TA) system compatible with part BBa_K899005. The TA system in this part contains mazF as a toxin and ccdA as antitoxin. It should be noted that since we put the antitoxin last (and the antitoxin degrades faster than the toxin) there should be a gtg start codon for the toxins in this part and BBa_K899006 and a ATG startcodon in front of the antitoxins. This should equalize the relationship between antitoxin and toxin molecules.

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Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 640
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 494
1000
COMPATIBLE WITH RFC[1000]

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 Heat inducible promoter K33801 regulating the expression of fructan:fructan fructosyltransferase (FFT) gene and a toxin-antitoxin (TA) system compatible with part BBa_K899005. The TA system in this part contains mazF as a toxin and ccdA as antitoxin. It should be noted that since we put the antitoxin last (and the antitoxin degrades faster than the toxin) there should be a gtg start codon for the toxins in this part and BBa_K899006 and a ATG startcodon in front of the antitoxins. This should equalize the relationship between antitoxin and toxin molecules.
+<img src="https://static.igem.org/mediawiki/2012/thumb/7/77/Giant_Bacteria%21fin%21_.png/800px-Giant_Bacteria%21fin%21_.png"
 <!-- Add more about the biology of this part here