Difference between revisions of "Part:BBa K861173:Experience"
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<br>The correct clones were cultured in 96-well plate at 37℃ for 24 hours,then the fluorescence and absorbance at 600 nm were recorded on a SpectraMax M2 plate reader.All fluorescence was normalized with absorbance at 600 nm.The cell density was characterized by the absorbance at 600nm,which increased with glucose concentration.The result is showed in the figure bellow. | <br>The correct clones were cultured in 96-well plate at 37℃ for 24 hours,then the fluorescence and absorbance at 600 nm were recorded on a SpectraMax M2 plate reader.All fluorescence was normalized with absorbance at 600 nm.The cell density was characterized by the absorbance at 600nm,which increased with glucose concentration.The result is showed in the figure bellow. | ||
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Latest revision as of 00:23, 27 September 2012
This experience page is provided so that any user may enter their experience using this part.
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how you used this part and how it worked out.
Applications of BBa_K861173
Our experiements was performed as bellow:
The correct clones were cultured in 96-well plate at 37℃ for 24 hours,then the fluorescence and absorbance at 600 nm were recorded on a SpectraMax M2 plate reader.All fluorescence was normalized with absorbance at 600 nm.The cell density was characterized by the absorbance at 600nm,which increased with glucose concentration.The result is showed in the figure bellow.
In this figure, the fluorescence of RFP generator promoted by PcstA decreased when concentration of glucose rose,that is to say,promoter PcstA can be activated by CRP at low glucose concentration.
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