Difference between revisions of "Part:BBa K778005"

Revision as of 00:08, 27 September 2012

RBS-fhlA363-d.term

FhlA-E363G generator without promoter (RBS-fhlAE363G-d.term)

FhlA is the trnascriptional activator of hycAp Part:BBa K778000 . E.coli overexpressing FhlA produce H2 (since hycAp is the promoter of FHL(formate hydrogen lyase)). fhlA-E363G has one point mutation (A to G, glutamic acid to glycine). This mutation increased H2 production under the conditions in the previous research (Viviana et.al.2009). However, in our research (team UT-Tokyo 2012[http://2012.igem.org/Team:UT-Tokyo/Project/H2_E.coli]), this mutation did not increase H2 production.

Detailed Information :[http://2012.igem.org/Team:UT-Tokyo/Project/H2_E.coli]

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 2115
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 1394
1000
COMPATIBLE WITH RFC[1000]

@@ Line 7: / Line 7: @@
 E.coli overexpressing FhlA produce H2 (since hycAp is the promoter of FHL(formate hydrogen lyase)).
 fhlA-E363G has one point mutation (A to G, glutamic acid to glycine).
+This mutation increased H2 production under the conditions in the previous research (Viviana et.al.2009).
+However, in our  research (team UT-Tokyo 2012[http://2012.igem.org/Team:UT-Tokyo/Project/H2_E.coli]), this mutation did not increase H2 production.
 Detailed Information :[http://2012.igem.org/Team:UT-Tokyo/Project/H2_E.coli]