Difference between revisions of "Part:BBa K812130"

 
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<partinfo>BBa_K812130 short</partinfo>
 
<partinfo>BBa_K812130 short</partinfo>
  
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Citrine gene flanked by a kozak sequence for eukaryotic expression in biobrick format. This is designed to be used in mammalian and ovarion expression systems such as PCS2+ part : K812000. Ready for immediate use in Xenopus.
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Citrine gene flanked by a kozak sequence for eukaryotic expression in biobrick format. This is designed to be used in mammalian and ovarion expression systems. The backbone of the plasmid is pCS2+ with the CMV promoter is <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K812000">BBa_K812000</a> and the J04450 is replaced by the mCitrine <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K812030">BBa_K812030</a>, it is ready for immediate use in Xenopus.
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</br>This part was characterized and the result is below.
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Tadpoles have 4 days (near stage 46) and the expression of mCitrine is localized in tail's muscles.
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<img src="https://static.igem.org/mediawiki/2012/b/b6/Picture_citrine_7.09.jpg" alt="perdu" width="500px" /><br/>
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</html>
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 00:05, 27 September 2012

Citrine reporter with a Kozak sequence for expression in Xenopus

Citrine gene flanked by a kozak sequence for eukaryotic expression in biobrick format. This is designed to be used in mammalian and ovarion expression systems. The backbone of the plasmid is pCS2+ with the CMV promoter is <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K812000">BBa_K812000</a> and the J04450 is replaced by the mCitrine <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K812030">BBa_K812030</a>, it is ready for immediate use in Xenopus.

</br>This part was characterized and the result is below.

Tadpoles have 4 days (near stage 46) and the expression of mCitrine is localized in tail's muscles. <img src="Picture_citrine_7.09.jpg" alt="perdu" width="500px" />

</html>


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 667
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]