Difference between revisions of "Part:BBa K812030"
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<partinfo>BBa_K812030 short</partinfo> | <partinfo>BBa_K812030 short</partinfo> | ||
| − | + | mCitrine gene flanked by a kozak sequence for eukaryotic expression in biobrick format. This part is in pSB1C3. | |
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| + | </br>This part was characterized in pCS2+ <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K812000">BBa_K812000</a> which contains the CMV promoter. </br> J04450 was replaced by this part, it built the BioBrick <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K812130">BBa_K812130</a>. This plasmid (<a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K812133">BBa_K812133</a>) express mCitrine into <i>Xenopus</i>: <br/> | ||
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| + | Tadpoles have 4 days (near stage 46) and the expression of mCitrine is localized in tail's muscles. | ||
| + | <img src="/wiki/images/b/b6/Picture_citrine_7.09.jpg" alt="perdu" width="500px" /><br/> | ||
| + | </html> | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Revision as of 23:54, 26 September 2012
mCitrine reporter with a Kozak sequence for eukaryotic expression
mCitrine gene flanked by a kozak sequence for eukaryotic expression in biobrick format. This part is in pSB1C3.
This part was characterized in pCS2+ BBa_K812000 which contains the CMV promoter. J04450 was replaced by this part, it built the BioBrick BBa_K812130. This plasmid (BBa_K812133) express mCitrine into Xenopus:
Tadpoles have 4 days (near stage 46) and the expression of mCitrine is localized in tail's muscles.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 667
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]