Difference between revisions of "Part:BBa K808010"
(→Usage and Biology) |
|||
| Line 4: | Line 4: | ||
tphB is coding for the dihydrodiol decarboxylase from ''Comamonas testosteroni Kf-1''. It´s catalyze the second reaction in the terephthalic acid degration. | tphB is coding for the dihydrodiol decarboxylase from ''Comamonas testosteroni Kf-1''. It´s catalyze the second reaction in the terephthalic acid degration. | ||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| Line 43: | Line 12: | ||
| + | ===Usage and Biology=== | ||
Revision as of 23:50, 26 September 2012
tphB: reaction from DCD to protocatechuate
Homology modelling of TphB. For simulation parameters http://2012.igem.org/Team:TU_Darmstadt/Modeling_Homologie_Modeling#TphB click here.
tphB is coding for the dihydrodiol decarboxylase from Comamonas testosteroni Kf-1. It´s catalyze the second reaction in the terephthalic acid degration.
Usage and Biology
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 155
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 202
Illegal NgoMIV site found at 877
Illegal AgeI site found at 939 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 76
References
- Sasoh, M., E. Masai, et al. (2006). "Characterization of the terephthalate degradation genes of Comamonas sp. strain E6." Appl Environ Microbiol 72(3): 1825-1832.
- Fukuhara, Y., K. Inakazu, et al. (2010). "Characterization of the isophthalate degradation genes of Comamonas sp. strain E6." Appl Environ Microbiol 76(2): 519-527.
- Kamimura, N., T. Aoyama, et al. (2010). "Characterization of the protocatechuate 4,5-cleavage pathway operon in Comamonas sp. strain E6 and discovery of a novel pathway gene." Appl Environ Microbiol 76(24): 8093-8101.
- Schläfli HR, Weiss MA, Leisinger T, Cook AM. (1994)"Terephthalate 1,2-dioxygenase system from Comamonas testosteroni T-2: purification and some properties of the oxygenase component." J Bacteriol. 1994 Nov;176(21):6644-52.