Difference between revisions of "Part:BBa K812031"
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| − | </br>This part was characterized in pCS2+ | + | </br>This part was characterized in pCS2+ <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K812000">BBa_K812000</a> which contains the CMV promoter . </br> J04450 was replaced by this part, it built the BioBrick <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K812133">BBa_K812133</a>. This plasmid (<a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K812133">BBa_K812133</a>) express sfGFP into Xenopus: <br/> |
<img src="https://static.igem.org/mediawiki/2012/2/27/PCS2%2B_sfGFP_20%2621_09.png" alt="perdu" width="880px" /> <br/><br/> | <img src="https://static.igem.org/mediawiki/2012/2/27/PCS2%2B_sfGFP_20%2621_09.png" alt="perdu" width="880px" /> <br/><br/> | ||
Revision as of 23:46, 26 September 2012
sfGFP with kozak sequence for eukaryotic expression
Super folded GFP fused with a kozak sequence to enhance its expression in eukaryotic cells. To be used with an eukaryotic expression system such as pSC2+ (BBa_K812000) and related parts. This part is in pSB1C3.
This part was characterized in pCS2+ BBa_K812000 which contains the CMV promoter . J04450 was replaced by this part, it built the BioBrick BBa_K812133. This plasmid (BBa_K812133) express sfGFP into Xenopus:
The expression of sfGFP is present only in one of the two embryo/tadpole. sfGFP is present only in few tissue as others reporters. sfGFP is expressed in tail's muscles and branchial basket for this tadpole. After injection of this plasmid into Xenopus it does not diffuse in the eggs, and the plasmid express sfGFP in few different tissues.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 19