Difference between revisions of "Part:BBa K812110"
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<partinfo>BBa_K812110 short</partinfo> | <partinfo>BBa_K812110 short</partinfo> | ||
| − | This part is a super folded GFP fused to an AID tag for its recignition by the ubiquitinase E3 that induces its degradation in the presence of auxin in the eukaryote cell. his part is in pCS2+, the plasmid backbone is <html><a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K812000">BBa_K812000</a></html> and the device is from <html><a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K812010">BBa_K812010</a></html> | + | This part is a super folded GFP fused to an AID tag for its recignition by the ubiquitinase E3 that induces its degradation in the presence of auxin in the eukaryote cell. his part is in pCS2+, the plasmid backbone is <html><a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K812000">BBa_K812000</a></html> and the device is from <html><a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K812010">BBa_K812010</a></html>. |
This part is a part of a device pattented by Kanemaki Masato, Kakimoto Tatsuo, Nishimura Kohei, Takisawa Haruhiko and Fukagawa Tatsuo for yeast and mammalian cells use (http://www.freepatentsonline.com/y2012/0115232.html). However the pattent does not cover the use for oviparian such as frogs and chicken. | This part is a part of a device pattented by Kanemaki Masato, Kakimoto Tatsuo, Nishimura Kohei, Takisawa Haruhiko and Fukagawa Tatsuo for yeast and mammalian cells use (http://www.freepatentsonline.com/y2012/0115232.html). However the pattent does not cover the use for oviparian such as frogs and chicken. | ||
Revision as of 23:27, 26 September 2012
GFP fusion with the ubuquitinase E3 OsTirI recoginition domain for PSC2+ plasmid
This part is a super folded GFP fused to an AID tag for its recignition by the ubiquitinase E3 that induces its degradation in the presence of auxin in the eukaryote cell. his part is in pCS2+, the plasmid backbone is BBa_K812000 and the device is from BBa_K812010.
This part is a part of a device pattented by Kanemaki Masato, Kakimoto Tatsuo, Nishimura Kohei, Takisawa Haruhiko and Fukagawa Tatsuo for yeast and mammalian cells use (http://www.freepatentsonline.com/y2012/0115232.html). However the pattent does not cover the use for oviparian such as frogs and chicken.
The GFP-AID contains 2 NLS domains that relocate the protein in the nucleus of the cells for a better signal concentration.
The tadpole 1 express GFP-aid in epidermic cells, whereas the tadpole 2 express GFP-aid in optical nerve,nostril nerve, tail muscle cells and branchial basket.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1020
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1405