Difference between revisions of "Part:BBa K808014"

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AroY is reported to catalyze the reaction of protocatechuate (3,4-Dihydroxybenzoate) to catechol under anaerobic conditions. This is the last step in the degradation of terephtalic acid to catechol. Gel permeation chromatography shows that AroY has a homopentomeric structure and a mass of 285.4 kDa (Figure 2).
 
AroY is reported to catalyze the reaction of protocatechuate (3,4-Dihydroxybenzoate) to catechol under anaerobic conditions. This is the last step in the degradation of terephtalic acid to catechol. Gel permeation chromatography shows that AroY has a homopentomeric structure and a mass of 285.4 kDa (Figure 2).
  
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[[Image:GPC_AroY.JPG|550px|thumb|left|Figure 2. '''GPC analysis of AroY'''. The Peak of AroY has a retention time of 33 minutes.]]
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[[Image:GPC_AroY.JPG|300px|thumb|right|Figure 2. '''GPC analysis of AroY'''. The Peak of AroY has a retention time of 33 minutes.]]
  
  
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[[Image:AroY_ff.jpg|550px|thumb|left|Figure 3. '''Funktional test for AroY:''' '''1)''' AroY with 50 mM Protocatechuate after 24 h; '''2)''' 1) after addition of XylE and 10 min inkubation.]]
 
  
  
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K808014 SequenceAndFeatures</partinfo>
  
  
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===Functional Parameters===
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<partinfo>BBa_K808014 parameters</partinfo>
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==Part Characterisation ==
  
  
  
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[[Image:AroY_ff.jpg|thumb|right|300px|Figure 3. '''Functional test for AroY:'''<br> '''1)''' AroY with 50 mM Protocatechuate after 24 h; <br>'''2)''' after addition of XylE and 10 min incubation]]
  
  
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===Functional Parameters===
 
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==References==
 
==References==

Revision as of 23:23, 26 September 2012

AroY: Catalyzes protocatechuate to catechol

Figure 1. Gaussian network modelling of AroY. The supposed membrane anchor in red. For simulation parameters http://2012.igem.org/Team:TU_Darmstadt/Modeling_GNM#AroY click here.

AroY codes for the protocatechuate deacarboxylase from Klebsiella pneumonie ssp. pneumonie. It has a molecular mass of 57 kDa and is reported to form a complex of five identical AroY subunits. [http://2012.igem.org/Team:TU_Darmstadt/Modeling_GNM#AroY Gaussian network modelling] showed a flexible helix linker. This could be either a membrane anchor or responsible for the formation of a pentamer.


Usage and Biology

AroY is reported to catalyze the reaction of protocatechuate (3,4-Dihydroxybenzoate) to catechol under anaerobic conditions. This is the last step in the degradation of terephtalic acid to catechol. Gel permeation chromatography shows that AroY has a homopentomeric structure and a mass of 285.4 kDa (Figure 2).

Figure 2. GPC analysis of AroY. The Peak of AroY has a retention time of 33 minutes.










Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 309
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 37



Part Characterisation

Figure 3. Functional test for AroY:
1) AroY with 50 mM Protocatechuate after 24 h;
2) after addition of XylE and 10 min incubation






References

  • He, Z. and J. Wiegel, Purification and characterization of an oxygen-sensitive, reversible 3,4-dihydroxybenzoate decarboxylase from Clostridium hydroxybenzoicum. J Bacteriol, 1996. 178(12): p. 3539-43.
  • Grant, D.J. and J.C. Patel, The non-oxidative decarboxylation of p-hydroxybenzoic acid, gentisic acid, protocatechuic acid and gallic acid by Klebsiella aerogenes (Aerobacter aerogenes). Antonie Van Leeuwenhoek, 1969. 35(3): p. 325-43.